keap1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc keap1

Deletion of microglial Sirt6 inhibited the NRF2-HO1 signaling and worsened the peroxidation damage. (A) Gene Ontology (GO) analysis was performed on RNA-Seq data from microglia sorted from Sirt6 MCKO and Sirt6 fl/fl control mice. (B) TAC, MDA, SOD, and GSH/GSSG levels at 5 days after LPS injection. n = 4 mice. (C) Gene Set Enrichment Analysis (GSEA) of RNA-Seq data profiled from microglia sorted from Sirt6 MCKO and Sirt6 fl/fl control mice. (D-E) Analysis of NRF2-HO1 and associated signaling proteins in sorted microglia. Protein levels of NRF2, <t>KEAP1,</t> HO-1, NQO1, NLRP3, Cleaved Caspase-3, and Cleaved IL-1β were assessed by Western blot (D) and quantified (E). n = 4 mice. Data are mean ± SEM. Statistical significance between two groups was determined by an unpaired two-tailed Student's t-test.

Keap1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+keap1/KEAP1+Rabbit+mAb/pmc13050088-51-35-37
Average 96 stars, based on 435 article reviews

keap1 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling"

Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling

Journal: Neurobiology of Stress

doi: 10.1016/j.ynstr.2026.100804

Figure Legend Snippet: Deletion of microglial Sirt6 inhibited the NRF2-HO1 signaling and worsened the peroxidation damage. (A) Gene Ontology (GO) analysis was performed on RNA-Seq data from microglia sorted from Sirt6 MCKO and Sirt6 fl/fl control mice. (B) TAC, MDA, SOD, and GSH/GSSG levels at 5 days after LPS injection. n = 4 mice. (C) Gene Set Enrichment Analysis (GSEA) of RNA-Seq data profiled from microglia sorted from Sirt6 MCKO and Sirt6 fl/fl control mice. (D-E) Analysis of NRF2-HO1 and associated signaling proteins in sorted microglia. Protein levels of NRF2, KEAP1, HO-1, NQO1, NLRP3, Cleaved Caspase-3, and Cleaved IL-1β were assessed by Western blot (D) and quantified (E). n = 4 mice. Data are mean ± SEM. Statistical significance between two groups was determined by an unpaired two-tailed Student's t-test.

Techniques Used: RNA Sequencing, Control, Injection, Western Blot, Two Tailed Test

other:

Article Title: Dietary Glyphosate Exposure Disrupts Hepatic and Reproductive Function in Female Zebrafish at Regulatory Safe Levels
Article Snippet: The primary antibodies and working dilutions employed for liver tissue were anti-xCT/SLC7A11 (1:50, D2M7A, Cell Signaling Technology, Danvers, MA, USA), anti-DMT1/SLC11A2 (1:500, D3V8G, Cell Signaling Technology, Danvers, MA, USA), and anti-Keap1 (1:100, D6B12, Cell Signaling Technology, Danvers, MA, USA).

Article Title: A novel antioxidant peptide DD12 (DWPDARGIWHND) derived from dry cured ham alleviates H2O2-induced oxidative damage via the Keap1-Nrf2-ARE pathway.
Article Snippet: This study investigated the antioxidant mechanism of the dry-cured ham-derived peptide DD12 and its regulatory effect on the Keap1-Nrf2-ARE signaling pathway.. Network pharmacological analysis showed that DD12 was associated with multiple antioxidant targets, and KEGG analysis revealed that it may regulate 112 signaling pathways, especially the Keap1-Nrf2 pathway.. PPI network analysis identified five key targets, and molecular docking demonstrated that DD12 binds to NFE2L2 with high affinity.

Article Title: Targeted KEAP1 Disruption Enhances Antioxidant Defense And Mesenchymal Stromal Cell Therapy For Chronic Limb-threatening Ischemia.
Article Snippet: Primary antibodies: anti-KEAP1 (Cell Signaling Technology, 354 #4678), anti-phospho-NRF2 (Abcam, #ab76026), anti- NRF2 (Cell Signaling Technology, 355 #12721), and β-actin (Cell Signaling Technology, #4970).

Article Title: Sappanchalcone suppresses NSCLC by oxidative stress-driven DNA damage and ER stress activation through PIEZO1 modulation
Article Snippet: anti-KEAP1 , Cell Signaling Technology , Cat#7705; RRID: AB_10860422.

Western Blot:

Article Title: 7,8-Dihydroxyflavone alleviates ulcerative colitis by binding keap1 to activate an slc7a11-dependent inhibition of NF-κB p65 signaling in macrophages.
Article Snippet: 30 μL Streptavidin agarose (P2159, Beyotime, China) was added to the cell lysates and incubated at 4°C for 4 h. The agaroses were washed with cold-PBS, boiled in protein loading buffer, and subjected to SDS-PAGE. .. This was followed by immunoblot analysis with anti-keap1 (1:500, 8047S, CST, USA) and anti-Nrf2 (1:1000, # 12721T, CST, USA) antibodies. .. After incubating with primary antibodies overnight at 4°C, PVDF were incubated with goat anti-rabbit (1:1000, A0208, Beyotime, China) for 1 h at room temperature.

Article Title: 7,8-Dihydroxyflavone alleviates ulcerative colitis by binding keap1 to activate an slc7a11-dependent inhibition of NF-κB p65 signaling in macrophages.
Article Snippet: .. This was followed by immunoblot analysis with anti-keap1 (1:1000, # 8047S, CST, USA) and anti-β-actin (1:1000, # 2118S, CST, USA) antibodies. ..

Control:

Article Title: Protein arginine methyltransferase 7-mediated arginine mono-methylation stabilizes SRY-box transcription factor 9 to promote non-small cell lung cancer progression
Article Snippet: .. Anti-T7-Tag (1:1000 dilution, Cat# 13246), anti-HA-Tag (1:1000 dilution, Cat# 3724), anti-GFP-Tag (1:1000 dilution, Cat# 2956), anti-His-Tag (1:1000 dilution, Cat# 12698), Rabbit (DA1E) mAb IgG XP® Isotype Control (Cat# 3900), Mouse (G3A1) mAb IgG1 Isotype Control (Cat# 5415), anti-SOX9 (1:1000 dilution, Cat# 82630), anti-PRMT7 (1:1000 dilution, Cat#14762), MMe-Arginine (1:1000 dilution, Cat# 8015S) and anti-KEAP1 (1:1000 dilution, cat# 8047) were purchased from Cell Signaling Technology. .. Anti-SOX9 (1:500 dilution, Cat# sc-166505), anti-PRMT7 (1:1000 dilution, Cat# sc-376077), anti-α-Tubulin (1:800 dilution, Cat# sc-23948) were purchased from Santa Cruz Biotechnology.

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